Fungal diversity and ex vitro symbiotic germination of Serapias vomeracea (Orchidaceae)

Conservation of orchids can be possible with effective seed germination and seedling growth methods. In this context, ex vitro symbiotic orchid seeds may convenient advantageous. study, both the diversity root endophytic fungi in Serapias vomeracea (Burm.f.) Briq. effects these on germination, development tuber formation were revealed. The isolated monthly for two years from S. roots isolates identified based morphological characters internal transcribed spacer (ITS) region nuclear ribosomal DNA (rDNA) sequences. All Rhizoctonia-like that joined mycorrhizal association closely related to Tulasnella calospora (thirty isolates). Non-Rhizoctonia are Fusarium tricinctum (two isolates), Aspergillus spelaeus (one isolate) Talaromyces pinophilus (Pezizales) isolate). viability rate was 90.32%. packs placed soils containing fungus process followed. associated promoted development. Isolate Svl 21 (Tulasnella sp.) found have highest (98%) but isolate 4 developed seedlings advanced leaves (stage (S4): and/or rooted, 13.67%). at S4 transferred natural environment; first tubers observed seven months after. time, a tuberous European orchid, adult plant environment.